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- Document 0347
- DOCN M94A0347
- TI Improved specificity of in vitro anti-HIV antibody production:
- implications for diagnosis and timing of transmission in infants born to
- HIV-seropositive mothers.
- DT 9412
- AU Wang XP; Paul M; Tetali S; Abrams E; Bamji M; Gulick L; Chirmule N;
- Oyaizu N; Bakshi S; Pahwa S; Department of Pediatrics, North Shore
- University Hospital-Cornell; University Medical College, New York, New
- York 11030.
- SO AIDS Res Hum Retroviruses. 1994 Jun;10(6):691-9. Unique Identifier :
- AIDSLINE MED/94355114
- AB In vitro anti-HIV antibody production (IVAP), initially introduced as a
- method for diagnosis of human immunodeficiency virus type 1 (HIV-1)
- infection in infants, has been limited in its application because of
- poor specificity and sensitivity early in life. The aims of this study
- were to improve the specificity of the IVAP assay and to evaluate its
- sensitivity in conjunction with assays of HIV culture, polymerase chain
- reaction (PCR), and p24 antigen. To prevent false-positive reactions
- resulting from maternal serum-derived cytophilic anti-HIV IgG,
- additional preculture and washing steps for peripheral blood mononuclear
- cells (PBMCs) were introduced that resulted in dramatic improvement in
- specificity of IVAP. The sensitivity of the revised IVAP at age < 3
- months in 20 infected infants was, however, only 25%; of 15 infected
- infants initially negative in IVAP, 13 became positive at a mean
- estimated age of 4.4 +/- 1.8 months. When correlated with virological
- assays, a failure to respond in IVAP at age < 1 month was often
- associated with negative virological identification, whereas a positive
- IVAP response at age < 3 months was always associated with positive
- results in all virological assays. Moreover, conversion from negative
- IVAP to positive responses occurred subsequent to, and not concurrently
- with, a positive virological identification of infected infants. The
- revised IVAP methodology renders this assay potentially useful as an
- additional tool not only for the diagnosis of HIV infection, but for
- estimating timing of maternal-infant HIV transmission as well.
- DE *Antibody Specificity Biological Markers Child, Preschool
- Enzyme-Linked Immunosorbent Assay Female Human *HIV Antibodies HIV
- Infections/*CONGENITAL/*DIAGNOSIS/TRANSMISSION
- HIV-1/*IMMUNOLOGY/ISOLATION & PURIF Infant Infant,
- Newborn/*MICROBIOLOGY Pregnancy Pregnancy Complications,
- Infectious/*DIAGNOSIS Support, U.S. Gov't, P.H.S. Time Factors
- JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
-
-